|
| Title : |
Lowcost Production of In Vitro Plantlets for Sustainable Pre-basic Seed Potato Production In Nepal |
| Material Type: |
printed text |
| Authors: |
Binesh M. Sakha, Author ; Gyan P. Rai, Author ; Shambhu P. Dhital, Author |
| Pagination: |
162-167 p. |
| Languages : |
English (eng) |
| Keywords: |
intensive culture, in vitro, mini tubers, pre-basic seed, sustainable |
| Abstract: |
In vitro and in vivo experiments were conducted in the tissue culture laboratory and
glasshouse of National Potato Research Programme, Khumaltar during 1997/98 to
minimize the cost of in vitro potato plantlets production. Use of sugar as substitute for
sucrose in culture showed no difference in the growth performance of in vitro potato
plantlets, and even in their yield performance under glasshouse condition. Culture of in
vitro potato plantlets on test tubes at the rate of 40 culture tubes per tray of 50 tubes
capacity was efficient than raising usual 30 culture tubes per tray, since both produced
more vigorous plantlets with higher nodal explants harvest as compared to culturing 50
tubes per tray. Culturing of 10 plantlets per bottle produced higher proportion of usable
nodal explants as compared to plantlets cultured at the rate of 12 and 15 plantlets per
bottle. Therefore, for the sustainable PBS production, sucrose could be substituted by
sugar in the cultures medium, in vitro potato plantlets subculture on trays at the rate of
40 tubes per tray and final multiplication of cultures on jam bottles at the rate .of 10
plantlets per bottle. A 37% cost reduction in the in vitro culture of potato was achieved
by intensive culture of plantlets on jam bottles. |
| Link for e-copy: |
http://nepaknol.org.np/narc/dl/?r=240 |
Lowcost Production of In Vitro Plantlets for Sustainable Pre-basic Seed Potato Production In Nepal [printed text] / Binesh M. Sakha, Author ; Gyan P. Rai, Author ; Shambhu P. Dhital, Author . - [s.d.] . - 162-167 p. Languages : English ( eng) | Keywords: |
intensive culture, in vitro, mini tubers, pre-basic seed, sustainable |
| Abstract: |
In vitro and in vivo experiments were conducted in the tissue culture laboratory and
glasshouse of National Potato Research Programme, Khumaltar during 1997/98 to
minimize the cost of in vitro potato plantlets production. Use of sugar as substitute for
sucrose in culture showed no difference in the growth performance of in vitro potato
plantlets, and even in their yield performance under glasshouse condition. Culture of in
vitro potato plantlets on test tubes at the rate of 40 culture tubes per tray of 50 tubes
capacity was efficient than raising usual 30 culture tubes per tray, since both produced
more vigorous plantlets with higher nodal explants harvest as compared to culturing 50
tubes per tray. Culturing of 10 plantlets per bottle produced higher proportion of usable
nodal explants as compared to plantlets cultured at the rate of 12 and 15 plantlets per
bottle. Therefore, for the sustainable PBS production, sucrose could be substituted by
sugar in the cultures medium, in vitro potato plantlets subculture on trays at the rate of
40 tubes per tray and final multiplication of cultures on jam bottles at the rate .of 10
plantlets per bottle. A 37% cost reduction in the in vitro culture of potato was achieved
by intensive culture of plantlets on jam bottles. |
| Link for e-copy: |
http://nepaknol.org.np/narc/dl/?r=240 |
|
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